Chymotrypsin

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Beijing Geyuantianrun Bio-tech Co.,Ltd

Business Type:Manufacturer

Country/Region:China

Ddu Verified

HOT Rank

8/10

Product Information

  • Pharmacopeia:CP
  • Shelf Life:18 months
  • Storage:Sealed

Description

Chymotrypsin

Source: Bovine (Porcine) pancreas

Chymotrypsin which is the endopeptidase extracted and separated from the fresh pancreas of bovine (or porcine).

Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule. It is white and light yellow crystal or amorphous powder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum PH is 8~9. It is stable at a dry state, but will be quickly deactivated in water solution, most stable in the water solution with PH3~4. When it acts on the protein, it will hydrolyze the peptide bond formed by the carboxyl of L-tyrosine and L-phenylalanine in priority.

The high purity Chymotrypsin of Beijing Geyuantianrun Bio-tech Co., Ltd.. is purified by re-crystallization, and then by Ion Exchange Chromatography and ultra-filtration.

Application

--In the clinical, it always used to general trauma, wound healing that after surgery, anti-inflammatory, prevent local edema, hematocele, sprain hematoma, partial swelling that after breast surgery, tympanitisrhinitis and so on. Use for the removal of cataract, Loose the lashes muscle ligament, reduce the cyst rupture and the hurt of retina.Besides, the combination use with the Trypsin and used as a broad-spectrum anti-inflammatory drugs.

--as the auxiliary medicine of antibiotic and antifungal drugs,it is widely used in  the field of animal medicine and animal health industry.

Specification

ItemsSpecificationMethod
AppearanceWhite or almost white lyophilized powderUSP33
IdentificationNo purple color develops within 3 minutesUSP33
Loss on dryingNo more than 5.0%USP33
Residue on IgnitionNo more than 2.5%USP33
Microbial limits:
Pseudomonas aeruginosa
Salmonella
Staphylococcus aureus
AbsentUSP33
TrypsinNo more than 1%USP33
AssayChymotrypsin≥1000 USPunits/mg powderUSP33
ItemsSpecificationStandard
DescriptionWhite crystalline lyophilized powderEP9.0
Identification1) A purple colour develops
2)No purple colour develops within 3 min of mixing
EP9.0
Appearance of solutionSolution S is not more opalescent than reference suspension ⅡEP9.0
Absorbance1) 281nm : 18.5 ~ 22.5
2) 250nm : not more than 8.0
EP9.0
PH3.0-5.0EP9.0
HistamineNot more than 1µg/5.0µkatalEP9.0
Loss on dryingNot more than 5%EP9.0
Limit of trypsinNot more than 1%EP9.0
Microbial limits:
Pseudomonas aeruginosa
Salmonella
AbsentEP9.0
AssayNot less than 5.0 ukatal/mg, calculated on the dried basisEP9.0

Storage

Sealed, Dark, at temperature 2-8℃


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